Gallery - Spirogyra
Maximum-intensity projection of a 3D widefield fluorescence image of spirogyra
- 0.75 NA Air objective lens at 540 nm
- 331nm XY pixels and 1μm-spaced Z slices
- 326 x 265 pixels and 25 slices
- Original data courtesy of Olympus Soft Imaging Solutions, Münster, Germany
Results from A Practical Guide to Deconvolution of Fluorescence Microscope Imagery, David S.C. Biggs, Microscopy Today, Volume18, Issue 01 Jan 2010, pp 10 - 14
Original
Nearest Neighbors
Wiener filter
MLE (10 iterations)
